3 ms·
PacBio reads don't extend to the million base length and are a considerably more expensive approach than short read sequencing if assembly is happening anyway.
by ejstronge 5y ago
PacBio reads don't extend to the million base length and are a considerably more expensive approach than short read sequencing if assembly is happening anyway.
ONT is a great tool but the goal of this report isn't necessarily to show that these molecules are 1 million bases in length vs showing that they represent novel DNA sequences.
In summary, ONT and PacBio are neither more appropriate or necessarily more direct methods here.
- andrewon 5y agoWould have to disagree. They will need to confirm these were not artifacts. Long read sequencing assembly is the best approach to show the computationally assembled reads indeed coming from single contiguous molecules.
- ejstronge 5y agoI'm not sure what kind of artifact would assemble into a series of novel ORFs but I suppose we will indeed have to disagree.