3 ms·
Long reads are still not as widely used since the error rates are much higher than in shotgun approach.
by f6v 5y ago
Long reads are still not as widely used since the error rates are much higher than in shotgun approach.
- tdido 5y agoActually, for the use case of genome assemblies you can compensate for the error rate with depth of coverage. Long reads are already the state-of-the-art for assembling genomes and are allowing us to get information that is simply invisible using short reads. https://www.biorxiv.org/content/10.1101/2021.05.26.445798v1 https://www.biorxiv.org/content/10.1101/2021.05.26.445798v1
- f6v 5y agoIt's definitely getting better, but errors have to be corrected computationally, and it still seems like a big challenge.
- londons_explore 5y agoOne would think a hybrid approach will bring the best results...? Long reads help with alignment and arrangement, and short reads eliminate small errors of just a few base pairs.