3 ms·
Yes. This type of error could be easily determined. If it's an contamination or an error, it will have very low read coverage (typical sequencing project be 50-
by noname123 5y ago
Yes. This type of error could be easily determined. If it's an contamination or an error, it will have very low read coverage (typical sequencing project be 50-200x meaning if you realign and pile up the reads back to the main assembly, you get a normal distribution centered around 50-200 reads supporting a particular base or region. If you have a very low coverage region or a sharp drop off in coverage, then it's most likely an error.
Metagenomics assembly also do additional binning prior to assembly based on the abundance and GC content of the cluster reads to separate out the different taxas of the sample. How well the read clusters are distanced by this huerisirc is another measure of quality of assembly.
- f6v 5y ago> If it's an contamination or an error, it will have very low read coverage (typical sequencing project be 50-200x meaning if you realign and pile up the reads back to the main assembly, you get a normal distribution centered around 50-200 reads supporting a particular base or region. If you have a very low coverage region or a sharp drop off in coverage, then it's most likely an error. Wouldn’t sequence-specific biases(capture efficiency, amplification) result in distortions?