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Yeah I meant RNA but it was early in the morning and my fingers autocorrected to mRNA. And many samples ARE degraded and only partial reads can be recovered.
by lamontcg 5y ago
Yeah I meant RNA but it was early in the morning and my fingers autocorrected to mRNA.
And many samples ARE degraded and only partial reads can be recovered.
> “These samples are not like huge vials of blood,” Rasmussen said. “It’s not like a big Erlenmeyer flask of green liquid.” Researchers would have to grow the virus in cells in order to stand a real chance of infecting people, she added, and it’s difficult to grow viruses from these swabbed samples even if you try to. There’s not much virus in them, and what you get tends to be contaminated with virus-killing detritus. “Technically it’s very challenging to directly isolate virus from field samples from wild animals. So that makes it unlikely that just handling those samples would result in some kind of infection.” Finally, Rasmussen added, the chemical solution that’s used to stabilize the viral RNA for sequencing is a very potent disinfectant its own right.
(from the title article)
PCR amplification means that you can find the needle in the haystack and find enough RNA to construct aa genome out of it. A lot of the time you only get partial reads or don't get the whole genome. There isn't enough viable virus there in order to infect anyone.
- native_samples 5y agoYou skipped over the second part of my response. I'll point it out again: "Researchers would have to grow the virus in cells in order to stand a real chance of infecting people" They were filing patents on designs for bat cages. Videos from inside the WIV before COVID showed live bats. They were doing far more than growing virus in cells, they were growing them in live animals, as virologists typically do. "PCR amplification means that you can find the needle in the haystack and find enough RNA to construct aa genome out of it" This would be relevant if virologists were mere lab technicians running PCR tests, but they aren't.