3 ms·
When it comes to the problem of targeting, one interesting and promising tech is photochemical internalisation [https://en.m.wikipedia.org/wiki/Photochemical_in
by espenwa 5y ago
When it comes to the problem of targeting, one interesting and promising tech is photochemical internalisation [https://en.m.wikipedia.org/wiki/Photochemical_internalization https://en.m.wikipedia.org/wiki/Photochemical_internalizatio...], where you put the mRNA inside photosensitive molecules (and not lipids) and then shine some light on the tissue/organ where you want the mRNA delivered. Where activated by the light, the molecules then enter the cells, dissolves and deliver the mRNA.
The Norwegian company PCI Biotech has a tech they call fimaNAc for doing this with naked mRNA.
https://www.pcibiotech.no/nucleicacids https://www.pcibiotech.no/nucleicacids
This presentation has a lot of illustrations and explanations:
https://www.pcibiotech.no/s/PCI-Biotech-SMi-RNA-Therapeutics-presentation-10-Feb-2021.pdf https://www.pcibiotech.no/s/PCI-Biotech-SMi-RNA-Therapeutics...
- monocasa 5y agoWhat happens to the unactivated mRNA in that case? I was under the impression that it was typically the actual use of the mRNA by the ribosomes that broke them down generally, but I could be off base there.
- gus_massa 5y agoThe same mRNA can be used by multiple ribosomes. More info and "photos": https://biology.stackexchange.com/questions/54752/can-mrna-be-used-by-ribosomes-more-than-once https://biology.stackexchange.com/questions/54752/can-mrna-b...
- monocasa 5y agoSure, it isn't destroyed immediately and the mRNA is used multiple time, but that's still ultimately the ribosomes damaging the mRNA from use. My question is around how they break down if there aren't ribosomes involved (ie. if the capsules above aren't opened because they weren't exposed to the light trigger).
- sudosysgen 5y agoEventually the capsule will break down, be filtered out or be destroyed by the immune system and any RNA left will be broken down by RNAse.
- gus_massa 5y agoMore info about RNA destruction and RNAse https://en.wikipedia.org/wiki/Ribonuclease https://en.wikipedia.org/wiki/Ribonuclease
- kmarc 5y agoThis is one of the most amazing things I've read lately (apart from mRNA based vaccination itself). Thanks for sharing.
- deleted 5y ago[deleted]