5 ms·

I am worried about the low level of intact mRNA in those vaccines, esp. as an IT guy. https://www.medicalnewstoday.com/articles/early-concerns-raised-over-leve

I am worried about the low level of intact mRNA in those vaccines, esp. as an IT guy. https://www.medicalnewstoday.com/articles/early-concerns-raised-over-levels-of-intact-mrna-in-pfizer-vaccine#Instructions-for-making-protein https://www.medicalnewstoday.com/articles/early-concerns-rai... 25-45% randomly modified or defective mRNA doesn't sound safe. It's a bit like machine code with random errors, isn't it?

wahern 5y ago
What's the replication accuracy of SARS-CoV-2?
- lnwlebjel 5y ago
  At least 95% I would wager
nabla9 5y ago
That's why there are clinical trials. Humans testing to find out what happens. All mRNA disappears from body in few days anyway.
pjscott 5y ago
RNA uses an error-correcting code, and if that fails all that should happen is that some of the proteins your ribosomes create will be misshapen. Your body is good at dealing with foreign proteins.
- tgb 5y ago
  What error correction does RNA have? Are you just referring to the third base in the codons? DNA has some but I don't know of any for RNA, and it hardly seems worth it for most organisms to have RNA error-correction: it's better to just degrade the RNA and retranscribe it.
  - fastaguy88 5y ago
    RNA and DNA essentially use the same genetic code, which is degenerate and thus somewhat error correcting.
    - tgb 5y ago
      You mean the degeneracy in the last base of codons that I mention? I wouldn't even call that an error correcting: would you count a file type that just skipped every third byte 'error correction'? I wouldn't. This isn't a robust system and most changes to any bases will change the protein sequence. Contrast that to DNA which does have error correction, since there are two strands instead of one and also two chromosomes with nearly identical information. Both are used to repair errors, at least some of the time. I'm not aware of such a system for RNA and it's not clear where it would get the information from - though maybe the most likely system would be a 'self-destruct' where errors are detected but since repair is impossible, the RNA is just destroyed.
      - fastaguy88 5y ago
        I completely agree. I was addressing a confusion of the previous poster.
- TeMPOraL 5y ago
  Except when it isn't. I'm sure this problem has been explored and ruled out, but as a general note, when I read that "all that should happen is some proteins will be misshapen", my mind immediately screams prions.
  - XorNot 5y ago
    Prions need to be beta-sheet proteins with specific conformations. They're hard to generate, and certainly are not single-base substitutions - otherwise the biosphere would be riddled with them.
    - seaman1921 5y ago
      Thanks, this is quite re-assuring since I am worried about prions as well. Could you please suggest a link for me to further educate myself on how unlikely this is ?
      - XorNot 5y ago
        This isn't bad: https://www.scientificamerican.com/article/what-is-a-prion-specifica/ https://www.scientificamerican.com/article/what-is-a-prion-s... The basic punchline is that while prion diseases are terrifying, there's not a lot of them and they are quite specific - you have to have a protein which is already able to adopt that conformational structure, and it has to be a protein we already make natively, as prions only work with what's there. We are probably still missing part of the picture as well: i.e. the prion diseases which cause problems are brain diseases, we don't see the same issue with other tissues. It would be difficult to actually induce a prion disease in someone via genetic vaccine in the first place, since prion's notably don't have a genetic component - and in the case of vCJD the infectious and benign protein sequences are the same (i.e. the coding is already something we carry, they're conformational jumps).
        seaman1921 5y ago
        > the coding is already something we carry, they're conformational jumps Aha, this is helpful. And I believe mRNAs are not known to trigger these conformational jumps. Another fact-check article supporting your statement: https://www.reuters.com/article/fact-check-no-evidence-that-pfizers-covi-idUSL1N2MZ382 https://www.reuters.com/article/fact-check-no-evidence-that-...
- seaman1921 5y ago
  > Your body is good at dealing with foreign proteins. Not always - Are you familiar with Prions diseases ?
  - lordlimecat 5y ago
    Prions are not foreign proteins. They have to be misfolded native proteins to cause disease.
deleted 5y ago
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wahern 5y ago

What's the replication accuracy of SARS-CoV-2?

lnwlebjel 5y ago
At least 95% I would wager

nabla9 5y ago

That's why there are clinical trials. Humans testing to find out what happens. All mRNA disappears from body in few days anyway.

pjscott 5y ago

RNA uses an error-correcting code, and if that fails all that should happen is that some of the proteins your ribosomes create will be misshapen. Your body is good at dealing with foreign proteins.

tgb 5y ago
What error correction does RNA have? Are you just referring to the third base in the codons? DNA has some but I don't know of any for RNA, and it hardly seems worth it for most organisms to have RNA error-correction: it's better to just degrade the RNA and retranscribe it.
- fastaguy88 5y ago
  RNA and DNA essentially use the same genetic code, which is degenerate and thus somewhat error correcting.
  - tgb 5y ago
    You mean the degeneracy in the last base of codons that I mention? I wouldn't even call that an error correcting: would you count a file type that just skipped every third byte 'error correction'? I wouldn't. This isn't a robust system and most changes to any bases will change the protein sequence. Contrast that to DNA which does have error correction, since there are two strands instead of one and also two chromosomes with nearly identical information. Both are used to repair errors, at least some of the time. I'm not aware of such a system for RNA and it's not clear where it would get the information from - though maybe the most likely system would be a 'self-destruct' where errors are detected but since repair is impossible, the RNA is just destroyed.
    - fastaguy88 5y ago
      I completely agree. I was addressing a confusion of the previous poster.
TeMPOraL 5y ago
Except when it isn't. I'm sure this problem has been explored and ruled out, but as a general note, when I read that "all that should happen is some proteins will be misshapen", my mind immediately screams prions.
- XorNot 5y ago
  Prions need to be beta-sheet proteins with specific conformations. They're hard to generate, and certainly are not single-base substitutions - otherwise the biosphere would be riddled with them.
  - seaman1921 5y ago
    Thanks, this is quite re-assuring since I am worried about prions as well. Could you please suggest a link for me to further educate myself on how unlikely this is ?
    - XorNot 5y ago
      This isn't bad: https://www.scientificamerican.com/article/what-is-a-prion-specifica/ https://www.scientificamerican.com/article/what-is-a-prion-s... The basic punchline is that while prion diseases are terrifying, there's not a lot of them and they are quite specific - you have to have a protein which is already able to adopt that conformational structure, and it has to be a protein we already make natively, as prions only work with what's there. We are probably still missing part of the picture as well: i.e. the prion diseases which cause problems are brain diseases, we don't see the same issue with other tissues. It would be difficult to actually induce a prion disease in someone via genetic vaccine in the first place, since prion's notably don't have a genetic component - and in the case of vCJD the infectious and benign protein sequences are the same (i.e. the coding is already something we carry, they're conformational jumps).
      - seaman1921 5y ago
        > the coding is already something we carry, they're conformational jumps Aha, this is helpful. And I believe mRNAs are not known to trigger these conformational jumps. Another fact-check article supporting your statement: https://www.reuters.com/article/fact-check-no-evidence-that-pfizers-covi-idUSL1N2MZ382 https://www.reuters.com/article/fact-check-no-evidence-that-...
seaman1921 5y ago
> Your body is good at dealing with foreign proteins. Not always - Are you familiar with Prions diseases ?
- lordlimecat 5y ago
  Prions are not foreign proteins. They have to be misfolded native proteins to cause disease.

deleted 5y ago

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