Check out BRENDA [1]. It's the best one I've found for annotated pathways. With regards to your tool idea, it is still very expensive to generate DNA from scra

Check out BRENDA [1]. It's the best one I've found for annotated pathways. With regards to your tool idea, it is still very expensive to generate DNA from scratch. Designing a theoretical plasmid won't get you all the way there if you can't source the parent DNA sequence. We usually pay ~$0.30/base for DNA synthesis. Plus, I'm not sure what the metabolic pathway of psilocybin is but you may need several plasmids to recreate the whole thing in E. coli (generally limited to ~10-15 KB). An interesting idea but definitely no small feat of engineering. [1] https://www.brenda-enzymes.org/pathway_index.php https://www.brenda-enzymes.org/pathway_index.php