free floating spike in vitro cultured human cells developed pathological state. when a spike protien is expressed as an insertion into the cytoplasmic membrane

free floating spike in vitro cultured human cells developed pathological state. when a spike protien is expressed as an insertion into the cytoplasmic membrane there is very limited mobility, and less potential to approach ACE2 with appropriate orientation and energy for receptor ligand-interaction to occur, this would result in somewhat less interaction of the spike with the rennin angiotensin system[RAS]. a concern would be the possible development of abberant or deletion type spike protiens translated from undesirerable spike-mRNA species, the scenario of most concern would be a spike protien with malformed or deleted transmembrane domains, thus allowing a speudo spike protien to move freely outside the host cell. this doesnt indicate that there is an unconceived problem, this publication is recognizing that perturbations of the RAS are highly consequential; a major part of covid pathology; and a hidden gotcha, should vaccine development ever become cavalier regarding RAS signaling and spike based agents