3 ms·
Optogenetic experiments with C. Elegans can be done at home. You can pick up cheap high-powered LEDs from online retailers. I think plasmid and viral vectors ar
by whymauri 5y ago
Optogenetic experiments with C. Elegans can be done at home. You can pick up cheap high-powered LEDs from online retailers. I think plasmid and viral vectors are within your price range of a few hundred dollars for the popular Chr2 strains. Here's a lab write-up from Caltech's freshman bio class:
http://bi1x.caltech.edu/2019/handouts/c_elegans_optogenetics.pdf http://bi1x.caltech.edu/2019/handouts/c_elegans_optogenetics...
It skips the process of getting your worms to express Chr2, but there's protocols to help with that:
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191802 https://journals.plos.org/plosone/article?id=10.1371/journal...