0.1X coverage is nowhere near enough to get any useful information. The Nanopore error rate is huge (10-15%), with substitutions, insertions and deletions all

0.1X coverage is nowhere near enough to get any useful information. The Nanopore error rate is huge (10-15%), with substitutions, insertions and deletions all common. The required coverage is more like 10X to 30X or more to get some accuracy in base calling and identifying variants. Far far better idea to just pay someone to do short read resequencing. Long read sequencing is great for assembling genomes and finding structural variants. Nanopore looks cheap in the first instance, but it's just not designed for this job. HiFi sequencing gives the best of both worlds - accuracy of short reads and decent length. https://www.pacb.com/smrt-science/smrt-sequencing/hifi-reads-for-highly-accurate-long-read-sequencing/ https://www.pacb.com/smrt-science/smrt-sequencing/hifi-reads... Not accessible to the home user though.