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Was this done using the knife edge microscopy technique or was each tissue sliced imaged after mechanically separating it from the rest?
by akiselev 5y ago
Was this done using the knife edge microscopy technique or was each tissue sliced imaged after mechanically separating it from the rest?
- mjanusz 5y agoThe latter. The tissue block is sliced first in the ATUM which collects the sections on tape. These are then mounted on silicon wafers, and imaged with the MultiSEM. Our preprint at https://www.biorxiv.org/content/10.1101/2021.05.29.446289v1 https://www.biorxiv.org/content/10.1101/2021.05.29.446289v1 has some more details about the process, including figures illustrating the ATUM and the tissue block. (I'm one of the authors of the paper)
- jeremybms 5y agoThis dataset was collected using physical ~33nm-thick sections that were cut with a diamond knife and stored (and preserved) on a carbon tape before imaging with a multi-beam scanning electron microscope. You can get the full details in the preprint paper: https://www.biorxiv.org/content/10.1101/2021.05.29.446289v1 https://www.biorxiv.org/content/10.1101/2021.05.29.446289v1