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Here is the part I didn't get--they are saying that a mutation of the spike protein causes the binding to epithelial cells. But the real virus, with correct spi

Here is the part I didn't get--they are saying that a mutation of the spike protein causes the binding to epithelial cells. But the real virus, with correct spike proteins, also causes this inflammation. So why does the vaccine only cause it as a result of the mutation? Why do the correctly produced proteins from the vaccine not also cause it?

maxerickson 5y ago
Edit: I'm wrong here. They are manufacturing errors more than they are mutations. Since the vaccine and virus use the same machinery to make the proteins, they both result in proteins with errors.
- spuz 5y ago
  But that is not the case according to the paper. The virus is RNA based whereas the vaccines at issue are DNA based. The problem as described by the paper is: > the viral piece of DNA [of Adenovirus based vaccines] - deriving from an RNA virus - is not optimized to be transcribed inside of the nucleus. ... Thus, it could well be that the Spike open reading frame of SARS-CoV-2 is potentially disrupted by arbitrary splice events when transcribed inside the nucleus. Most, if not all, of these undesirable splice events would produce shorter protein variants, ... leading to soluble Spike protein variants. So the error happens during DNA to RNA transcription in the nucleus. The Covid-19 virus is RNA based so how are the same splicing events occurring when it replicates itself in our cells? Edit: There is further mention in the paper of predicting and then detecting potential splice sites in the "wildtype Spike" (which I think means the RNA of the spike protein of the actual virus) and they found several potential splice sites, but not as many as those predicted / found in the AZ and J&J vaccines. It's still unclear how those splice sites could be activated if the virus replicates entirely outside the nucleus but there is probably some information I'm missing here.
  - maxerickson 5y ago
    Yeah, you are right. It's that the vaccine spikes don't stick the way they are intended to, so they are moving around the body like the virus.
deleted 5y ago
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greenwich26 5y ago
The real spike proteins are insoluble and stick to certain membranes, like in your lungs. The mutant ones are shorter and more soluble, so they can more easily accumulate elsewhere, e.g. the blood vessels in your brain: hence the vaccine-induced brain blood clots.

maxerickson 5y ago

Edit: I'm wrong here. They are manufacturing errors more than they are mutations. Since the vaccine and virus use the same machinery to make the proteins, they both result in proteins with errors.

spuz 5y ago
But that is not the case according to the paper. The virus is RNA based whereas the vaccines at issue are DNA based. The problem as described by the paper is: > the viral piece of DNA [of Adenovirus based vaccines] - deriving from an RNA virus - is not optimized to be transcribed inside of the nucleus. ... Thus, it could well be that the Spike open reading frame of SARS-CoV-2 is potentially disrupted by arbitrary splice events when transcribed inside the nucleus. Most, if not all, of these undesirable splice events would produce shorter protein variants, ... leading to soluble Spike protein variants. So the error happens during DNA to RNA transcription in the nucleus. The Covid-19 virus is RNA based so how are the same splicing events occurring when it replicates itself in our cells? Edit: There is further mention in the paper of predicting and then detecting potential splice sites in the "wildtype Spike" (which I think means the RNA of the spike protein of the actual virus) and they found several potential splice sites, but not as many as those predicted / found in the AZ and J&J vaccines. It's still unclear how those splice sites could be activated if the virus replicates entirely outside the nucleus but there is probably some information I'm missing here.
- maxerickson 5y ago
  Yeah, you are right. It's that the vaccine spikes don't stick the way they are intended to, so they are moving around the body like the virus.

deleted 5y ago

[deleted]

greenwich26 5y ago

The real spike proteins are insoluble and stick to certain membranes, like in your lungs. The mutant ones are shorter and more soluble, so they can more easily accumulate elsewhere, e.g. the blood vessels in your brain: hence the vaccine-induced brain blood clots.