3 ms·

Lipid nanoparticle delivery vehicle is really simplifying a lot of therapeutics. Looking forward to seeing Moderna and Acuitas continue to build out the platfo

Lipid nanoparticle delivery vehicle is really simplifying a lot of therapeutics. Looking forward to seeing Moderna and Acuitas continue to build out the platform for more targeted or effective actuator delivery.

ChaitanyaSai 5y ago
What are some good books to learn more about this? Thanks.
- alextheparrot 5y ago
  Sadly, I don't have a book to recommend. Most of the targeting today is happening through antibodies, but the majority of LNP delivery at this point is done passively without targeting mechanisms on the LNP (Needle injection point aside). That's actually one advantage of CRISPR over just injecting mRNA - you can target specific cell-lines (Even if you change the genome of many cell types) by using cell-specific promoters for the edits you make instead of relying on surface affinity based targeting of the LNP. Interesting resources: [0]: https://www.nature.com/articles/nrd.2017.243 https://www.nature.com/articles/nrd.2017.243 (General review 1) [1]: https://www.frontiersin.org/articles/10.3389/fmolb.2020.587997/full https://www.frontiersin.org/articles/10.3389/fmolb.2020.5879... (General review 2) [2]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498813/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498813/ (PCSK9 target again, but via siRNA actuator) [3]: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0715-0 https://genomebiology.biomedcentral.com/articles/10.1186/s13... (Example of cell-specific CRISPR integration)
  - ChaitanyaSai 5y ago
    Thanks for the pointers! A big fan of birds-eye-view books that let readers then follow individual trees. I guess these take time to put together and some fields (like genomics / genetic engineering, are too fast moving now?)
    - mncharity 5y ago
      > A big fan of birds-eye-view books Not a book, but the News Features in Nature have a similar flavor, eg [1]. And for following up with the motivating article... well, there's sci-hub, for now. [1] "The mysterious microbes that gave rise to complex life" https://www.nature.com/articles/d41586-021-01316-0 https://www.nature.com/articles/d41586-021-01316-0 in https://www.nature.com/nature/volumes/593/issues/7859 https://www.nature.com/nature/volumes/593/issues/7859 .
    - Duller-Finite 5y ago
      The Code Breaker by Walter Isaacson is a recent biography of Jennifer Doudna, who won the Nobel Prize for her pioneering work on CRISPR, that you might enjoy
    - alextheparrot 5y ago
      I’ve been recommending The Gene to people who are just generally interested in this sorta thing

ChaitanyaSai 5y ago

What are some good books to learn more about this? Thanks.

alextheparrot 5y ago
Sadly, I don't have a book to recommend. Most of the targeting today is happening through antibodies, but the majority of LNP delivery at this point is done passively without targeting mechanisms on the LNP (Needle injection point aside). That's actually one advantage of CRISPR over just injecting mRNA - you can target specific cell-lines (Even if you change the genome of many cell types) by using cell-specific promoters for the edits you make instead of relying on surface affinity based targeting of the LNP. Interesting resources: [0]: https://www.nature.com/articles/nrd.2017.243 https://www.nature.com/articles/nrd.2017.243 (General review 1) [1]: https://www.frontiersin.org/articles/10.3389/fmolb.2020.587997/full https://www.frontiersin.org/articles/10.3389/fmolb.2020.5879... (General review 2) [2]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498813/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498813/ (PCSK9 target again, but via siRNA actuator) [3]: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0715-0 https://genomebiology.biomedcentral.com/articles/10.1186/s13... (Example of cell-specific CRISPR integration)
- ChaitanyaSai 5y ago
  Thanks for the pointers! A big fan of birds-eye-view books that let readers then follow individual trees. I guess these take time to put together and some fields (like genomics / genetic engineering, are too fast moving now?)
  - mncharity 5y ago
    > A big fan of birds-eye-view books Not a book, but the News Features in Nature have a similar flavor, eg [1]. And for following up with the motivating article... well, there's sci-hub, for now. [1] "The mysterious microbes that gave rise to complex life" https://www.nature.com/articles/d41586-021-01316-0 https://www.nature.com/articles/d41586-021-01316-0 in https://www.nature.com/nature/volumes/593/issues/7859 https://www.nature.com/nature/volumes/593/issues/7859 .
  - Duller-Finite 5y ago
    The Code Breaker by Walter Isaacson is a recent biography of Jennifer Doudna, who won the Nobel Prize for her pioneering work on CRISPR, that you might enjoy
  - alextheparrot 5y ago
    I’ve been recommending The Gene to people who are just generally interested in this sorta thing