3 ms·
Causes instability when you transfer into yeast as your amplification medium; deleting the TE boosts the correct colony ratio from 50% to nearly 100%. I also i
by dnautics 5y ago
Causes instability when you transfer into yeast as your amplification medium; deleting the TE boosts the correct colony ratio from 50% to nearly 100%. I also identified the gene responsible for the phenomenon: it's RAD42. So if you're ever cloning new genomes that don't have TEs removed yet you might consider using RAD42- yeast.