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also transposons certainly exist in prokarya too, and probably drive innovation there as well, possibly assisting horizontal gene transfer. When I was in the s
by dnautics 5y ago
also transposons certainly exist in prokarya too, and probably drive innovation there as well, possibly assisting horizontal gene transfer. When I was in the synthetic genome lab at the venter institute, one of the first things I did was win a bet about the orientation of a transposable element in the syn1.0 genome that was eventually removed from the genome (the bet was not important enough to merit me being on the paper)
- wombatmobile 5y agoWhy was the TE removed from the syn1.0 genome?
- dnautics 5y agoCauses instability when you transfer into yeast as your amplification medium; deleting the TE boosts the correct colony ratio from 50% to nearly 100%. I also identified the gene responsible for the phenomenon: it's RAD42. So if you're ever cloning new genomes that don't have TEs removed yet you might consider using RAD42- yeast.