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For my understanding: crisper works by cutting the dna at specific points surrounding the relevant part and then exchanging, right? How likely is it, that in th
by doubtfuluser 6y ago
For my understanding: crisper works by cutting the dna at specific points surrounding the relevant part and then exchanging, right?
How likely is it, that in the whole DNA string there are other instances this “surrounding” matches and would be cut? How is this prevented / not causing side effects?
- julienreszka 6y agoDNA has something like "Bytes" Clustered Regularly Interspaced Short Palindromic Repeats
- yarcob 6y agoThe nice thing about CRISPR is that you can use pretty long sequences for matching, so in theory you can just pick a sequence that doesn't occur anywhere else and cut there. (Before CRISPR, you would use restriction enzymes with a short, fixed recognition sequence, typically 4-6 base pairs [1]. In that case it was a bit tricky to find the right enzyme that cuts the DNA in the right place, and not in an unwanted place. And you couldn't just cut the DNA in any place you want.) [1]: https://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_A https://en.wikipedia.org/wiki/List_of_restriction_enzyme_cut...