JCVI-Syn3A has been around for a while. Here is the genome sequence from 2018 - https://www.ncbi.nlm.nih.gov/nuccore/1241355755 https://www.ncbi.nlm.nih.gov/nuc

JCVI-Syn3A has been around for a while. Here is the genome sequence from 2018 - https://www.ncbi.nlm.nih.gov/nuccore/1241355755 https://www.ncbi.nlm.nih.gov/nuccore/1241355755 Back at FreeGenes we synthesized all of the genes from this organism with codon optimization for Escherichia coli (https://stanford.freegenes.org/collections/gene-sets/products/jcvi_syn3-0#description https://stanford.freegenes.org/collections/gene-sets/product...), the workhorse of synthetic biology. If you combine the right transcriptional and translational elements, you should be able to build a fully modular genome from this gene set! We also did a couple other organisms in pursuit of this modular genome. As a nice bonus, you can also easily do in-vitro cell-free experiments since the codon tables are nice (and I've heard from Kate Adamala's group that JCVI-Syn3 has pretty bad cell free, though that was a couple years ago) It's important to note that JCVI-Syn3A has a LOT of problems when it comes to its practical use. There just isn't enough energy being put into making an understandable and practical modular/minimal genome.