4 ms·
Meh, they probably just used lipofectamine (which has been around since the 90s) or something very similar. https://en.wikipedia.org/wiki/Lipofectamine https:/
by subroutine 6y ago
Meh, they probably just used lipofectamine (which has been around since the 90s) or something very similar.
https://en.wikipedia.org/wiki/Lipofectamine https://en.wikipedia.org/wiki/Lipofectamine
https://www.thermofisher.com/us/en/home/brands/product-brand/lipofectamine.html https://www.thermofisher.com/us/en/home/brands/product-brand...
- Duller-Finite 6y agolipofectamine is used for in vitro transfection, not in vivo gene delivery. The vaccines use lipid nanoparticles rather than liposomes
- MuffinFlavored 6y agoIf they didn't use lipofectamine, what did they most likely use?
- flobosg 6y agoDerek Lowe discussed it a few months ago: https://blogs.sciencemag.org/pipeline/archives/2021/01/11/rna-vaccines-and-their-lipids https://blogs.sciencemag.org/pipeline/archives/2021/01/11/rn... https://blogs.sciencemag.org/pipeline/archives/2021/02/22/more-on-mrna-vaccine-manufacturing https://blogs.sciencemag.org/pipeline/archives/2021/02/22/mo...
- neuronic 6y agoI absolutely do not have a link, but I remember reading that the lipid nanoparticles are actually created by mechanical action (possibly fluid dynamics/turbulence). I thought that was pretty neat.