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What are the purple and blue sections after the stop codon for? I read a little about the 3' region, but for the vaccine, are these sections taken from a partic
by kart23 6y ago
What are the purple and blue sections after the stop codon for? I read a little about the 3' region, but for the vaccine, are these sections taken from a particular natural human sequence, or specially engineered for something else?
- layoutIfNeeded 6y agoThat’s the copyright notice.
- iso1337 6y agoIt's the 3' (3-prime) UTR (un-translated region). It can affect the translation of the mRNA. https://en.wikipedia.org/wiki/Three_prime_untranslated_region#:~:text=In%20molecular%20genetics%2C%20the%20three,post%2Dtranscriptionally%20influence%20gene%20expression https://en.wikipedia.org/wiki/Three_prime_untranslated_regio.... The next thing is the poly-A tail: https://en.wikipedia.org/wiki/Polyadenylation https://en.wikipedia.org/wiki/Polyadenylation blasting the 3' UTR, we see it ~50% of it was copied from the human mitochondria tldr, extra regulatory signals (often not well understood)
- deleted 6y ago[deleted]
- dnautics 6y agoDoesn't really make sense for it to copy from the mitochondrion; I presume this thing gets expressed in the cytosol.
- fabian2k 6y agoThe 3' and 5' untranslated regions are the parts of the mRNA directly before and after the part that encodes the actual protein. So they are themselves not translated into amino acids. What they actually do can vary, but essentially they can provide places for other things to bind and influence what happens with the mRNA. There are some fancier cases like riboswitches, but you don't see those in humans. The stuff at the start and end of the mRNA also determines stability of the mRNA.
- flobosg 6y agoIn the BioNTech/Pfizer mRNA, the 3' (or the latter) half of the 3'-UTR comes from human mitochondrial 12S rRNA. The Moderna one has the 3'-UTR of the alpha subunit of human hemoglobin.
- hfjfktmtkrn 6y agoAmong other things that purple region determines the "priority"/"intensity" of the whole sequence. You want it as high as possible to make as much spike protein as possible. It's proprietary information, mostly they try various possibilities until they find one with high expression.
- Asparagirl 6y agoSo it’s the mRNA version of !important in CSS?
- hfjfktmtkrn 6y agoMore like font-weight, since !important is binary and this is more of a gradient.
- _theory_ 6y agoThat's the part that makes you randomly yell, "Hail Bill Gates!"