3 ms·
Wow! The 3D-Sim of the nuclear envelope at the wikipedia article was amazing but seeing the structure in the neuron is astounding. Do you know how long imaging
by fluidcruft 6y ago
Wow! The 3D-Sim of the nuclear envelope at the wikipedia article was amazing but seeing the structure in the neuron is astounding. Do you know how long imaging takes for this? I assume the post-processing is slow but will video be possible someday?
- nnmg 6y agoI am least familiar with SIM, but you can do live-cell SIM imaging for sure. The processing is a bit computationally intensive but not so bad (and can always be done post-hoc). The big thing you have to look out for is the light intensity killing the cells or bleaching your signals. One of our collaborators is actively working on on-the-fly SIM processing for live cell imaging. A quick glance at pubmed it looks like 11Hz was do-able several years ago https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/ and this (sorry paywalled, I've heard sci-hub has the paper...) https://pubmed.ncbi.nlm.nih.gov/30478322/ https://pubmed.ncbi.nlm.nih.gov/30478322/ STED is promising for live imaging too. Lots of beautiful pictures out there!