4 ms·
This only works up to a point before diffraction sets in and the picture quality starts to very noticeably suffer, and comes nowhere close to the depth of field
by devb 6y ago
This only works up to a point before diffraction sets in and the picture quality starts to very noticeably suffer, and comes nowhere close to the depth of field acquired with focus stacking. In fact, with stacking, the lens should be stopped down to the best f-stop anyway (f/8-11).
- namibj 6y agoGood (Vis/NIR) microscope optics go up to f/0.56 (NA 0.9) without oil immersion. Granted, such an objective will cost you 3.5k$ [0], but they don't go to these lengths without expecting near-diffraction-limited performance. [0]: https://www.thorlabs.com/thorproduct.cfm?partnumber=RMS60X-PFC https://www.thorlabs.com/thorproduct.cfm?partnumber=RMS60X-P...