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Thank you for the practical insight. I suspected that NanoPores are not just yet geared towards hobbyists. I happen to have some bioinformatics knowhow so it's
by Ovah 6y ago
Thank you for the practical insight. I suspected that NanoPores are not just yet geared towards hobbyists. I happen to have some bioinformatics knowhow so it's mainly a matter of hardware for me. As both you, u/bioinformatics and u/searine mention it is the overhead cost of flow cells etc that worries me from a hobbyist point of view.
- danpalmer 6y agoYep the flow cells are expensive, but more generally saying the hardware costs $1000 is probably more of a technicality – almost all sales are bigger contracts, with training, support, flow cells, hardware, included. Also that's great you have some bioinformatics experience, but are you sure it will apply here? I'm very unknowledgeable about this so forgive me if I'm wrong, but I believe that traditional machines do short-reads, whereas Nanopore does long-reads, which I believe invalidates many techniques for reconstructing the data, the tools, etc. This might not be something you have to be concerned about depending on the level you're working at, I don't know (maybe this is all "solved" by the time you're doing analysis?).
- Ovah 6y agoI think theoretical/practical background will allow me to figure stuff out as I go with relative ease. Often times in bioinformatics there are useful vignettes a Google search away. But you are right that specialized tools and libraries which I haven't used before are likely needed. At least for me the main bottleneck will be proper space for processing and the aspect of (reusable) hardware to keep costs down costs. While NanoPores appear to have hidden costs it still seems to be way cheaper than other methods if I want to have my own sequencing rig.