Not sure if they still do it this way, but some thirty years back we use to treat B-cells with Epstein Barr Virus (EBV) to cause them to immortalize. You basic

Not sure if they still do it this way, but some thirty years back we use to treat B-cells with Epstein Barr Virus (EBV) to cause them to immortalize. You basically shredded the red blood cells and hit the white blood cells with a heavy concentration of EBV. From there, you would look for antibody production for what you were after and then isolate those cells as they replicated.