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Impressive. > Basically those slices are taken at 10 microns and we are > working on fixing some of the problems (when you cut a > brain that thin you en
by apl 15y ago
Impressive.
> Basically those slices are taken at 10 microns and we are
> working on fixing some of the problems (when you cut a
> brain that thin you end up with a lot of tears and
> deformations)
Can you talk about how you deal with the inherent deformations? I've spent hundreds of hours on cryostats preparing rodent brain slices for autoradiography, and getting usable ones at 15-20 microns is a painful process. Should be even worse if you need to retain morphological information...
- nkassis 15y agoI'm probably not the best person to explain the whole process but I can put you in contact with the person who's working on it. (My email is in my profile). I'll try to explain what I know: For the most major tears and breaks we've actually had to fix them ourselves (Some were quite awefull, for example part of the right hemisphere on one slice broke off and was put back upside down ;p). For the less major thing, they can be fixed by looking at the surrounding slices, this is also how the slice are realigned, it's an iterative process where you take chunks of slices and aligned them then increase the chunks etc. At least that's what I gather. (Software developer talking about Brain imaging stuff is a bad idea ;p) EDIT: Also as for the morphological information I'm pretty sure that some of it will be affected by the process but I don't know how much. Our interface will provide the original and modified slices and a heat map of changes for comparaison in those cases. I don't know how that can be avoided.