I've worked with nucleic acids (DNA and RNA) in my postdoc, and now in my own lab, and I'm quite shocked that anyone would rely on UV absorption measurements. T

I've worked with nucleic acids (DNA and RNA) in my postdoc, and now in my own lab, and I'm quite shocked that anyone would rely on UV absorption measurements. They're inherently insensitive, and suffer from unreliability and reagent interference. Especially when they were taken over PCR based methods. It's a remarkable f*ck up for a clinical trial. These decisions should have been made with data from gold standard analytical methods.