4 ms·

Thanks for the additional links. I guess you would need temperature stable viral RNApol to do direct 'RNA' amplification. Not sure if that exists or if it's eve

Thanks for the additional links. I guess you would need temperature stable viral RNApol to do direct 'RNA' amplification. Not sure if that exists or if it's even possible due to RNA instability. As you say not so worthwhile due to high errors. Producing RNA from DNA amplicons (PCR products) makes sense, I'm curious if that is the source of material from in the vaccines, or if it's artificially synthesized? Would that be a trade secret?

rolph 6y ago
the exact sequences could be proprietary secrets. when you have determined a sequence of RNA corresponding to a desired protien you may construct a DNA template with modification to suit your purpose. the DNA polymerase used for DNA PCR is originally from a high tempurature tolerant organism, [thermophillic bcterium] and it is possible via searching high and low to find an organism that bears high temperature tolerant rdRNApol https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/thermophilic-bacterium https://www.sciencedirect.com/topics/biochemistry-genetics-a... and BTW the original inventor of PCR [kary mullis] was awake AF on LSD when he conceived of this technology while sitting on a park bench. https://en.wikipedia.org/wiki/Kary_Mullis https://en.wikipedia.org/wiki/Kary_Mullis i believe the original PCR was performed MANUALLY! with glassware and perfectionist tedium. https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinogens https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinoge... you may find this to be interesting: https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing
- innomatics 6y ago
  Thanks for the Kary Mullis story. I performed thousands of PCRs and even made some batches of taq many years ago but never knew about its ideation! I knew some pretty out there biochemists though.

rolph 6y ago

the exact sequences could be proprietary secrets. when you have determined a sequence of RNA corresponding to a desired protien you may construct a DNA template with modification to suit your purpose. the DNA polymerase used for DNA PCR is originally from a high tempurature tolerant organism, [thermophillic bcterium] and it is possible via searching high and low to find an organism that bears high temperature tolerant rdRNApol https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/thermophilic-bacterium https://www.sciencedirect.com/topics/biochemistry-genetics-a... and BTW the original inventor of PCR [kary mullis] was awake AF on LSD when he conceived of this technology while sitting on a park bench. https://en.wikipedia.org/wiki/Kary_Mullis https://en.wikipedia.org/wiki/Kary_Mullis i believe the original PCR was performed MANUALLY! with glassware and perfectionist tedium. https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinogens https://en.wikipedia.org/wiki/Kary_Mullis#Use_of_hallucinoge... you may find this to be interesting: https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing https://en.wikipedia.org/wiki/Ion_semiconductor_sequencing

innomatics 6y ago
Thanks for the Kary Mullis story. I performed thousands of PCRs and even made some batches of taq many years ago but never knew about its ideation! I knew some pretty out there biochemists though.