5 ms·
> We further observed that expression of Chi3l1 is regulated by the circadian clock, as deletion of the core clock proteins BMAL1 or CLOCK/NPAS2 strongly suppre
by zamfi 6y ago
> We further observed that expression of Chi3l1 is regulated by the circadian clock, as deletion of the core clock proteins BMAL1 or CLOCK/NPAS2 strongly suppresses basal Chi3l1 expression, whereas deletion of the negative clock regulators PER1/PER2 increased Chi3l1 expression. Basal Chi3l1 mRNA was nonrhythmic because of a long mRNA half-life in astrocytes. However, inflammatory induction of Chi3l1 was gated by the clock
I'm struck by how much this description sounds like someone debugging or reverse-engineering some unknown microcontroller.
Do you (or anyone) know: has molecular bio always been like this? Or are there new tools that allow for selective inhibition of signals to observe effects?
- sradman 6y agoThey use CRISPR/Cas9 [1] techniques on animal models, mice in this case. They knock out the gene that transcribes the protein in question and observe the impact in the lab. [1] https://en.wikipedia.org/wiki/CRISPR https://en.wikipedia.org/wiki/CRISPR
- hertzrat 6y agoIn this situation, what does disrupted circadian cycle refer to? Somebody who cannot sleep when they are tired and needs a lot of coffee in the morning? Or somebody who gets woken up a lot?