4 ms·
Hmm, but then the b-factor (and therefore also resolution) is dependent on the flexibility of molecule you want to look at? Does that mean that these guys just
by edna314 6y ago
Hmm, but then the b-factor (and therefore also resolution) is dependent on the flexibility of molecule you want to look at? Does that mean that these guys just found a system for which the main chain was rigid enough such that the thermal fluctuations are low enough?
- natechols 6y agoOh absolutely, they picked a model system that is both very rigid and has extremely high internal symmetry, and I assume they did rotational averaging to use fewer images. But these ideal systems are very useful for determining the limitations of the underlying technology like electron detectors; 15 years ago the best you could do with a molecule like this was around 4 Angstrom resolution.
- edna314 6y agoOk, that’s fair. But, then the number makes more sense in relative terms than in absolute. Still, absolutely impressive.