4 ms·
I remember when those vague blobs were a monumental achievement. It’s very cool to see these kinds of technologies break through and get refined like this.
by steve_adams_86 6y ago
I remember when those vague blobs were a monumental achievement. It’s very cool to see these kinds of technologies break through and get refined like this.
- chrisbrandow 6y agoIn terms of either sample prep or data processing, how time consuming is this relative to X-ray crystallography? Setting aside entirely the enormous benefit of not needing crystals. I’m a (former) chemist that has only ever had to use X-ray crystallography for small molecules. I’m just trying to get a sense of what the practice of doing this is like?
- natechols 6y agoI do not know what it's like in practice now, but on a purely theoretical basis, if you are trying to solve a completely novel structure with no known homologs, especially if it's something on the scale of a ribosome, direct imaging is always going to be easier than trying to solve the phase problem, both experimentally and computationally.