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The preprint Rapid, field-deployable nucleobase detection and identification using FnCas9 [1]: > Here, we present FnCas9 Editor Linked Uniform Detection Assay
by sradman 6y ago
The preprint Rapid, field-deployable nucleobase detection and identification using FnCas9 [1]:
> Here, we present FnCas9 Editor Linked Uniform Detection Assay (FELUDA)... At the heart of such a detection procedure lies the property of CRISPR proteins to accurately bind to target DNA or RNA, undergo conformational changes leading to cleavage of targets generating a reporter-based signal outcome.
> We then developed a pipeline to adapt FELUDA for an affinity-based fluorescent read-out system, where the amplification step generates biotinylated products which can then be immobilized on magnetic streptavidin beads.
The end-to-end process is similar to PCR and requires specialists to collect samples that are then processed by a lab.
[1] https://www.biorxiv.org/content/10.1101/2020.04.07.028167v2 https://www.biorxiv.org/content/10.1101/2020.04.07.028167v2