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Wow, thank you for that, I had no idea PCR is this inaccurate. The process always created a very "pure" image in my head, and now I feel kind of stupid never ch
by H8crilA 6y ago
Wow, thank you for that, I had no idea PCR is this inaccurate. The process always created a very "pure" image in my head, and now I feel kind of stupid never checking how inaccurate it is in practice. Of course I'm not a professional in the area.
How does it happen on a molecular level that PCR delivers a false positive? Are the primers sometimes broken (matching not exactly what they're supposed to match)?
- khuey 6y agoA properly performed PCR test is essentially 100% specific. False positives come from contamination of samples, mislabelled samples, and other forms of human error.
- deleted 6y ago[deleted]
- mehrdadn 6y ago> Wow, thank you for that, I had no idea PCR is this inaccurate. I'm not sure it really is. Take it with a grain of salt for now. I feel like there's a missing piece of the puzzle here.