4 ms·

So how does this one work? Live adeno’s?

aphextron 6y ago
>So how does this one work? Live adeno’s? Yes. It uses a live (weakened) chimpanzee adenovirus to insert the spike protein. https://www.drugs.com/history/azd1222.html https://www.drugs.com/history/azd1222.html
- mrfusion 6y ago
  Do the adenos actually have to infect the cells to produce the spikes?
  - dogma1138 6y ago
    Yes, they don’t replicate virons that could be assembled the virus was modified to mainly replicate the spike proteins since your cells send samples of anything they replicate to the cell wall for your immune system to monitor and sample this is how the adaptive immune response is achieved.
    - kurthr 6y ago
      Exactly, for strong long term response you want T-cell involvement.
    - im3w1l 6y ago
      > cells send samples of anything they replicate to the cell wall for your immune system to monitor Wow nature is truly a marvel! How do they do that? Or where could I read more?
      - deleted 6y ago
        [deleted]
    - nextos 6y ago
      > Wow nature is truly a marvel! How do they do that? Or where could I read more? Read about the HLA/MHC class I pathway. And also about JAK/STAT1, which is how cells decide if they are infected.
ethbr0 6y ago
The chimpanzee adenovirus is used as a vector, with its payload replaced with instructions to manufacture SARS-CoV-2 spike protein. Virus enters body, hopefully survives immune system long enough to insert instructions into your cells, your cells churn out tons of copies of coronavirus spike protein, your immune system sees the surfeit of spike protein and develops a response (antibodies and T* cells). https://blogs.sciencemag.org/pipeline/archives/2020/04/23/a-close-look-at-the-frontrunning-coronavirus-vaccines-as-of-april-23 https://blogs.sciencemag.org/pipeline/archives/2020/04/23/a-...
- mrfusion 6y ago
  This sounds pretty harmless. Any idea how they got a bad adverse event out of it?
  - ethbr0 6y ago
    Imagine a 4 billion year old codebase, written by dice throws and unit testing. The immune system is a "complicated" thing to work with.
    - Daynil 6y ago
      I'm going to print this out and frame it.
      - qchris 6y ago
        Yeah, I just wrote this down to remember for later. What a quote.
        ethbr0 6y ago
        Repressed humour from the programmer son of a pathologist. My favorite dad quip was 'Biology itself is proof against an intelligent designer.'
        6y ago
karmicthreat 6y ago
Thought Emporium has a decent video where he modifies an Adenovirus to inject the lactase gene into the cells of his intestinal tract. https://www.youtube.com/watch?v=J3FcbFqSoQY https://www.youtube.com/watch?v=J3FcbFqSoQY

>So how does this one work? Live adeno’s? Yes. It uses a live (weakened) chimpanzee adenovirus to insert the spike protein. https://www.drugs.com/history/azd1222.html https://www.drugs.com/history/azd1222.html

mrfusion 6y ago
Do the adenos actually have to infect the cells to produce the spikes?
- dogma1138 6y ago
  Yes, they don’t replicate virons that could be assembled the virus was modified to mainly replicate the spike proteins since your cells send samples of anything they replicate to the cell wall for your immune system to monitor and sample this is how the adaptive immune response is achieved.
  - kurthr 6y ago
    Exactly, for strong long term response you want T-cell involvement.
  - im3w1l 6y ago
    > cells send samples of anything they replicate to the cell wall for your immune system to monitor Wow nature is truly a marvel! How do they do that? Or where could I read more?
    - deleted 6y ago
      [deleted]
  - nextos 6y ago
    > Wow nature is truly a marvel! How do they do that? Or where could I read more? Read about the HLA/MHC class I pathway. And also about JAK/STAT1, which is how cells decide if they are infected.

ethbr0 6y ago

The chimpanzee adenovirus is used as a vector, with its payload replaced with instructions to manufacture SARS-CoV-2 spike protein. Virus enters body, hopefully survives immune system long enough to insert instructions into your cells, your cells churn out tons of copies of coronavirus spike protein, your immune system sees the surfeit of spike protein and develops a response (antibodies and T* cells). https://blogs.sciencemag.org/pipeline/archives/2020/04/23/a-close-look-at-the-frontrunning-coronavirus-vaccines-as-of-april-23 https://blogs.sciencemag.org/pipeline/archives/2020/04/23/a-...

mrfusion 6y ago
This sounds pretty harmless. Any idea how they got a bad adverse event out of it?
- ethbr0 6y ago
  Imagine a 4 billion year old codebase, written by dice throws and unit testing. The immune system is a "complicated" thing to work with.
  - Daynil 6y ago
    I'm going to print this out and frame it.
    - qchris 6y ago
      Yeah, I just wrote this down to remember for later. What a quote.
      - ethbr0 6y ago
        Repressed humour from the programmer son of a pathologist. My favorite dad quip was 'Biology itself is proof against an intelligent designer.'
        6y ago

karmicthreat 6y ago

Thought Emporium has a decent video where he modifies an Adenovirus to inject the lactase gene into the cells of his intestinal tract. https://www.youtube.com/watch?v=J3FcbFqSoQY https://www.youtube.com/watch?v=J3FcbFqSoQY