4 ms·
I don't think your percentage is correct. It depends on the PCRs target gene, the quality of the samples and the quality of the machine used. They are using rev
by wesleywt 6y ago
I don't think your percentage is correct. It depends on the PCRs target gene, the quality of the samples and the quality of the machine used. They are using reverse transcriptase quantitative PCR. What is your source for that FNR?
- wittyreference 6y agoAi et al. https://pubmed.ncbi.nlm.nih.gov/32101510/ https://pubmed.ncbi.nlm.nih.gov/32101510/ analysis of 1014 patients in Wuhan; Yang et al. looked at another 213 in Guangdong. Kucirka et al (https://www.acpjournals.org/doi/10.7326/M20-1495 https://www.acpjournals.org/doi/10.7326/M20-1495) did a pooled analysis of seven other studies and found that "over the 4 days of infection before the typical time of symptom onset (day 5), the probability of a false-negative result in an infected person decreases from 100% (95% CI, 100% to 100%) on day 1 to 67% (CI, 27% to 94%) on day 4. On the day of symptom onset, the median false-negative rate was 38% (CI, 18% to 65%). This decreased to 20% (CI, 12% to 30%) on day 8 (3 days after symptom onset) then began to increase again, from 21% (CI, 13% to 31%) on day 9 to 66% (CI, 54% to 77%) on day 21."