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From the methods: > SARS-CoV-2 viral load quantification: SARS-CoV-2 RNA levels were quantified with a quantitative viral load assay using the US CDC 2019-nCoV
by lbeltrame 6y ago
From the methods:
> SARS-CoV-2 viral load quantification: SARS-CoV-2 RNA levels were quantified with a quantitative viral load assay using the US CDC 2019-nCoV_N1 primers and probe set as previously described(10).
RNA. This can mean that they have a higher viral load, but high level of viral RNA does not necessarily equate with live virus. Why didn't they try to infect Vero E6 cells with the swab samples? (My guess: lack of equipment). Given the day of sampling, it may be still related, but given studies in children are far and few between, this is an IMO glaring omission.
I checked the paper for cell cultures or in vitro assays for live virus, no dice.
I'm kind of disappointed. We're well into the pandemic and we still rely on the presence of RNA alone to infer infectiousness? Why didn't the reviewers ask for that?