Reordering of oligos is expensive, which makes people not reproduce. Sanger sequencing is pretty expensive ($5 sure, but over 5kb on 3 different constructs beco

Reordering of oligos is expensive, which makes people not reproduce. Sanger sequencing is pretty expensive ($5 sure, but over 5kb on 3 different constructs becomes pricey). PCR machines also ain’t that cheap (when you’re a noob and don’t know what to buy), especially when you need to do more than 8 PCRs at a time. In theory, works in practice, and in practice, works well in theory. Even for experienced DIYbio folks, cloning isn’t just 1,2,3. Also, PCRs themselves aren’t very reproducible. It’s not about the overlapping sequence.