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How is gibson ivory tower? It's literally just based on overlapping sequences. And how is it hard to reproduce? You select out colonies and verify with sanger.
by vikramkr 6y ago
How is gibson ivory tower? It's literally just based on overlapping sequences. And how is it hard to reproduce? You select out colonies and verify with sanger. These are not things that are inaccessible to most people, especially if you live near a DIY bio hacker space, and if you get involved in the biohacker community you'll get a sense of what providers ship to residential address, where to get certain equiptment, and so on. Just googling will get you information that can help you figure out how to get what you need.
https://medium.com/@ThatMrE/a-guide-to-diybio-updated-2019-abd0956cdf74 https://medium.com/@ThatMrE/a-guide-to-diybio-updated-2019-a...
- koeng 6y agoReordering of oligos is expensive, which makes people not reproduce. Sanger sequencing is pretty expensive ($5 sure, but over 5kb on 3 different constructs becomes pricey). PCR machines also ain’t that cheap (when you’re a noob and don’t know what to buy), especially when you need to do more than 8 PCRs at a time. In theory, works in practice, and in practice, works well in theory. Even for experienced DIYbio folks, cloning isn’t just 1,2,3. Also, PCRs themselves aren’t very reproducible. It’s not about the overlapping sequence.