Slow organism was synechococcus, fast organism was E. Coli, original results were highly likely to carry over since it was a fundamental alteration to the enzym

Slow organism was synechococcus, fast organism was E. Coli, original results were highly likely to carry over since it was a fundamental alteration to the enzyme function. https://pubmed.ncbi.nlm.nih.gov/23819621/ https://pubmed.ncbi.nlm.nih.gov/23819621/ If you use the right tools and live in the right place you can get an e coli design cycle down to 48 hours. In San Diego, i could design primers in the afternoon, have them on my desk in the morning, do a pcr/Gibson assembly that was done by lunch, pick colonies in the evening, miniprep and retransform early the next day and have phenotypes soon after. It's also robust. I trained an intern with only classroom experience in molecular biology and he made 50 mutations and biochemically tested half over the course of 10 weeks. https://pubmed.ncbi.nlm.nih.gov/24934472/ https://pubmed.ncbi.nlm.nih.gov/24934472/