3 ms·
> I had a similar experience, and switching model organisms took my design cycle from 2 weeks to two days, and got huge results out of it. We then put the resul
by highfrequency 6y ago
> I had a similar experience, and switching model organisms took my design cycle from 2 weeks to two days, and got huge results out of it. We then put the results back in the original organism and recapitulated the improvement. Then my project got cancelled.
Very cool. If you care to elaborate on this: what were the two organisms, what made the second amenable to much faster design cycles, and how did you know that the results would likely carry over to the original organism?
- dnautics 6y agoSlow organism was synechococcus, fast organism was E. Coli, original results were highly likely to carry over since it was a fundamental alteration to the enzyme function. https://pubmed.ncbi.nlm.nih.gov/23819621/ https://pubmed.ncbi.nlm.nih.gov/23819621/ If you use the right tools and live in the right place you can get an e coli design cycle down to 48 hours. In San Diego, i could design primers in the afternoon, have them on my desk in the morning, do a pcr/Gibson assembly that was done by lunch, pick colonies in the evening, miniprep and retransform early the next day and have phenotypes soon after. It's also robust. I trained an intern with only classroom experience in molecular biology and he made 50 mutations and biochemically tested half over the course of 10 weeks. https://pubmed.ncbi.nlm.nih.gov/24934472/ https://pubmed.ncbi.nlm.nih.gov/24934472/