3 ms·
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797993/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797993/ "Despite predictions from both structure-based mode
by wrycoder 6y ago
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797993/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4797993/
"Despite predictions from both structure-based modeling and pseudotyping experiments, SHC014-MA15 was viable and replicated to high titers in Vero cells (Supplementary Fig. 2b). Similar to SARS, SHC014-MA15 also required a functional ACE2 molecule for entry, but uses human, civet, and bat orthologs (Supplementary Fig. 2c, d). To test the ability of SHC014 spike to mediate infection of the human airway, we examined 2B4 Calu-3 cells, a human epithelial airway cell line8, and found robust SHC014-MA15 replication comparable to SARS-CoV Urbani (Fig. 1c). To extend these findings, primary human airway epithelial cultures (HAEs) were infected and indicated robust replication of both viruses (Fig. 1d)."
They constructed a virus and tested in vitro on human cells and found robust replication. The exact chimeric virus they created would cause human disease if it escaped.
Note that Zheng-li Zhi is co-author. She is a virologist associated with the Whuhan lab. As the paper states, she supplied the sequences for the spike protein.
It is hard to believe this article would be ignored "because Trump".
- yters 6y agoThere might be a deeper reason these articles are being ignored.