2 ms·

I can't read the article due to paywall. How did they achieve this thermostability? Supercharging the surface?

• HarryHirsch 7y ago
  Site-saturation mutagenesis in the active site followed by introduction of disulphide bridges.
  • dnautics 7y ago
    Man. I would have guessed that adding disulfide bridges would decrease catalytic conversiom rates by making the protein too rigid.