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The exciting thing is that there is real progress in increasing temperature stability of enzymes. That's the point of the article, engineering an enzyme that is
by HarryHirsch 6y ago
The exciting thing is that there is real progress in increasing temperature stability of enzymes. That's the point of the article, engineering an enzyme that is still active above the glass transition temperature of the substrate. I remember a paper where they tried random mutations all over some enzyme, it was just mindless make-work for an army of graduate students. This one is different.
- dnautics 6y agoI can't read the article due to paywall. How did they achieve this thermostability? Supercharging the surface?
- HarryHirsch 6y agoSite-saturation mutagenesis in the active site followed by introduction of disulphide bridges.
- dnautics 6y agoMan. I would have guessed that adding disulfide bridges would decrease catalytic conversiom rates by making the protein too rigid.