3 ms·

I love the raise of open source / hobbyist experimental science. I have two used qPCR machines at home (which I bought for $450), and I have established relatio

I love the raise of open source / hobbyist experimental science. I have two used qPCR machines at home (which I bought for $450), and I have established relationships with Sigma-Aldrich and other consumables providers, so I now can e.g. run my own qPCR tests for SARS-CoV-2 RNA presence, as well as many other experiments.

anfractuosity 7y ago
That sounds really interesting, have you written up any of your experiments anywhere?
- atemerev 7y ago
  Not yet. I play to make a full report when I will receive a final component (low ROX master mix).
100ideas 7y ago
What’s your approach for getting covid19 template dna for the positive control reaction?
- atemerev 7y ago
  Just bought the positive control RNA template, it is included in most kits.
100ideas 7y ago
For those out there interested in DIY molecular diagnostics, in addition to qPCR, also check out this new technique: "SHERLOCK-based one-step test provides rapid and sensitive COVID-19 detection: New CRISPR-based research tool delivers results in an hour in a one-step reaction, advancing the technology closer to a point-of-care or at-home testing tool." - https://mcgovern.mit.edu/2020/05/05/sherlock-based-one-step-test-provides-rapid-and-sensitive-covid-19-detection/ https://mcgovern.mit.edu/2020/05/05/sherlock-based-one-step-... "STOPCovid is an effort to develop point-of-care and at-home tests for COVID-19. Developed by scientists at MIT, the McGovern Institute, and the Broad Institute, our test relies on a number of enzyme innovations to enable rapid detection in less than an hour without any complex instrumentation. We are committed to providing simple and easy-to-use tests that can be used in any setting so that society can reopen. On our website, we will provide updates as well as live clinical data from within our collaborator network. We are committed to working with partners to validate and distribute test kits. Please reach out to us (STOPcovid@mit.edu) if you are interested in working with us!" https://www.stopcovid.science https://www.stopcovid.science

anfractuosity 7y ago

That sounds really interesting, have you written up any of your experiments anywhere?

atemerev 7y ago
Not yet. I play to make a full report when I will receive a final component (low ROX master mix).

100ideas 7y ago

What’s your approach for getting covid19 template dna for the positive control reaction?

atemerev 7y ago
Just bought the positive control RNA template, it is included in most kits.

100ideas 7y ago

For those out there interested in DIY molecular diagnostics, in addition to qPCR, also check out this new technique: "SHERLOCK-based one-step test provides rapid and sensitive COVID-19 detection: New CRISPR-based research tool delivers results in an hour in a one-step reaction, advancing the technology closer to a point-of-care or at-home testing tool." - https://mcgovern.mit.edu/2020/05/05/sherlock-based-one-step-test-provides-rapid-and-sensitive-covid-19-detection/ https://mcgovern.mit.edu/2020/05/05/sherlock-based-one-step-... "STOPCovid is an effort to develop point-of-care and at-home tests for COVID-19. Developed by scientists at MIT, the McGovern Institute, and the Broad Institute, our test relies on a number of enzyme innovations to enable rapid detection in less than an hour without any complex instrumentation. We are committed to providing simple and easy-to-use tests that can be used in any setting so that society can reopen. On our website, we will provide updates as well as live clinical data from within our collaborator network. We are committed to working with partners to validate and distribute test kits. Please reach out to us (STOPcovid@mit.edu) if you are interested in working with us!" https://www.stopcovid.science https://www.stopcovid.science