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The paper provides strong evidence that the test is not a false positive. They show an amplification plot where you can easily see the positive sample and negat
by ttul 7y ago
The paper provides strong evidence that the test is not a false positive. They show an amplification plot where you can easily see the positive sample and negative sample along with the patient sample. The patient sample starts ramping up after cycle 30 of the PCR amplification process.
They support the COVID-19 diagnosis by looking at the patient's lung CT scan, which "revealed bilateral ground glass opacity in inferior lobes."
A decent explanation of PCR amplification can be found here: https://www.promega.ca/resources/guides/nucleic-acid-analysis/pcr-amplification/ https://www.promega.ca/resources/guides/nucleic-acid-analysi...
"Each cycle of PCR includes steps for template denaturation, primer annealing and primer extension. The initial step denatures the target DNA by heating it to 94°C or higher for 15 seconds to 2 minutes. In the denaturation process, the two intertwined strands of DNA separate from one another, producing the necessary single-stranded DNA template for replication by the thermostable DNA polymerase. In the next step of a cycle, the temperature is reduced to approximately 40–60°C. At this temperature, the oligonucleotide primers can form stable associations (anneal) with the denatured target DNA and serve as primers for the DNA polymerase. This step lasts approximately 15–60 seconds. Finally, the synthesis of new DNA begins as the reaction temperature is raised to the optimum for the DNA polymerase. For most thermostable DNA polymerases, this temperature is in the range of 70–74°C. The extension step lasts approximately 1–2 minutes. The next cycle begins with a return to 94°C for denaturation."