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The article mentioned using bar-coding to run pooled tests - that sounds really interesting. Anyone know if there's a link to a paper on that? The article doesn
by vikramkr 7y ago
The article mentioned using bar-coding to run pooled tests - that sounds really interesting. Anyone know if there's a link to a paper on that? The article doesn't link to anything on that unfortunately but that sounds really interesting. I'm trying to visualize how that works with qpcr, where the barcode is attached and where the primers bind (and presuambly after amplification you sanger them to read the barcode?).
- jamessb 7y agoHere's one preprint: https://docs.google.com/document/d/1kP2w_uTMSep2UxTCOnUhh1TMCjWvHEY0sUUpkJHPYV4/edit https://docs.google.com/document/d/1kP2w_uTMSep2UxTCOnUhh1TM... ("A Massively Parallel COVID-19 Diagnostic Assay for Simultaneous Testing of 19200 Patient Samples") I think Figure 1b is the diagram you want.
- vikramkr 7y agoAwesome! Thanks for finding that. So they do give up the extra specificity from the reverse primer by having it be a constant region in the barcode, I guess having a sanger step makes up for that a bit though since you can read what it ligated to past the barcode and see if it's a SARS-COV-2 fragment, but that does slow down getting the results. Like a truck of hard drives barreling down a street versus sending data over fiber. Get a lot of reads in giant batches or get less reads but with less latency.
- ziga 7y agoHere’s a paper from the Broad Institute: https://www.biorxiv.org/content/10.1101/2020.04.06.025635v1 https://www.biorxiv.org/content/10.1101/2020.04.06.025635v1