4 ms·
Isn't the dilution of the virus a problem if most of the patients are negative? You were using a test on a sample containing 100% virus positive blood, but now
by anticycle 7y ago
Isn't the dilution of the virus a problem if most of the patients are negative? You were using a test on a sample containing 100% virus positive blood, but now you are using a test on a sample that contains x% virus positive blood, with x potentially small
- pwoolf 7y agoTrue, dilution is a challenge but the assays used for COVID19 detection generally have a pretty wide dynamic range (3-5 orders of magnitude). The multiplex designs in Origami Assays mix between 4 (for S3) and 36 (for XL3) samples per well, depending on design. This means that samples are diluted by a max of 1:36 in the largest XL3 design (2.8% original concentration) so the assay used must be able to handle that. Alternatively, samples may be concentrated depending on the specific assay.