4 ms·
Wow is the least comprehensible (to me) comment I’ve ever seen on HN. Well played!
by freetime2 7y ago
Wow is the least comprehensible (to me) comment I’ve ever seen on HN. Well played!
- AstralStorm 7y agoI'll try to explain below. Monoclonal antibodies, or mAb are made usually by fusing a right B cell or splicing a gene sequence for the right light chains (V, D) into a leukemic cancer cell line producing a so called hybridoma. Those cells are damaged in such a way as to produce tons of antibodies and are immortal. By using a different starter cell line you can get different antibody classes. Also by providing a different interleukin stimulus to the cells. Typically IgG is made because it's easiest, but there are options. See: https://www.sciencedirect.com/science/article/abs/pii/S0022175915001246?via%3Dihub https://www.sciencedirect.com/science/article/abs/pii/S00221... Here's a helpful blog post: https://www.tebu-bio.com/blog/2018/11/13/monoclonal-antibodies-all-you-need-to-know-about-antibody-generation/ https://www.tebu-bio.com/blog/2018/11/13/monoclonal-antibodi...
- freetime2 7y agoFascinating... thanks for the additional info!
- DoofusOfDeath 7y agoTo be fair, the first two stanzas in that post are the most poetic I've ever read on HN.