4 ms·
It's the light chain that binds, you can modify heavy chain to do what you need and said. This is what's done when humanizing antibodies too. The extra infect
by AstralStorm 7y ago
It's the light chain that binds, you can modify heavy chain to do what you need and said.
This is what's done when humanizing antibodies too.
The extra infection potential is when the virus or bacteria can survive or thrive inside macrophages or monocytes, which react to opsonized particles, such as with antibodies.
You can make IgA or IgM instead to prevent this mechanism. They work by polymerization to bind whatever they attach to. Former is typically not activating the rest of immune system, while IgM would activate everything strongly perhaps inducing your own production.
- freetime2 7y agoWow is the least comprehensible (to me) comment I’ve ever seen on HN. Well played!
- AstralStorm 7y agoI'll try to explain below. Monoclonal antibodies, or mAb are made usually by fusing a right B cell or splicing a gene sequence for the right light chains (V, D) into a leukemic cancer cell line producing a so called hybridoma. Those cells are damaged in such a way as to produce tons of antibodies and are immortal. By using a different starter cell line you can get different antibody classes. Also by providing a different interleukin stimulus to the cells. Typically IgG is made because it's easiest, but there are options. See: https://www.sciencedirect.com/science/article/abs/pii/S0022175915001246?via%3Dihub https://www.sciencedirect.com/science/article/abs/pii/S00221... Here's a helpful blog post: https://www.tebu-bio.com/blog/2018/11/13/monoclonal-antibodies-all-you-need-to-know-about-antibody-generation/ https://www.tebu-bio.com/blog/2018/11/13/monoclonal-antibodi...
- freetime2 7y agoFascinating... thanks for the additional info!
- DoofusOfDeath 7y agoTo be fair, the first two stanzas in that post are the most poetic I've ever read on HN.