3 ms·
I looked at their one of their patents and it looks like they might be using something like rolling circle amplification instead of traditional amplification te
by vikramkr 7y ago
I looked at their one of their patents and it looks like they might be using something like rolling circle amplification instead of traditional amplification techniques for faster speed (I don't know off sure but it's one of the options in their claims). Kind of like LAMP pcr where you can amplify really quickly to get a signal really rapidly (although the dna at the end isn't really useable for further synthetic biology - it just provides a strong signal). They also mention a couple other types in this patent like strand displacement etc, so there's a lot of options they could be using.
Edit: the keyword to look for might be "isothermal" amplification, which means same - temperature amplification. Again, no clue if that's what they're using, I just see that they have patents on using isothermal techniques. These allow for amplifying DNA without cycling the temperature, usually letting the reaction accelerate quickly until a signal is detected, and it can accelerate really quickly sometimes. You know those videos of supercooled liquid water where you bump the bottle and the whole thing freezes? Imagine that you have a situation like that where adding non-coronavirus dna bumps the bottle incorrectly and nothing happens, while adding coronavirus dna makes the whole thing freezes. It's a rapid signal that just gets generated, but often (like with lamp) you just end up with a polymerizated mess that you can't use for DNA engineering in synthetic biology which is why you might use your temp. cycling methods for those uses. Also, they're good for telling you if something is or isn't there, but not so good at telling you how much dna you started with. To figure that out you again use a cycling reaction with the amount of dna doubling each step until it levels out so you know how many doublings happened and can calculate backwards (quantitative rt-pcr, or qPCR).
https://patents.google.com/patent/US8900828 https://patents.google.com/patent/US8900828
https://en.m.wikipedia.org/wiki/Rolling_circle_replication https://en.m.wikipedia.org/wiki/Rolling_circle_replication
https://en.m.wikipedia.org/wiki/Loop-mediated_isothermal_amplification https://en.m.wikipedia.org/wiki/Loop-mediated_isothermal_amp...
- tyre 7y agoThank you for the informative comment! Your example of the water bottle is perfect
- vikramkr 7y agoThanks! I was trying to find a universally known example or party trick of something just polymerizing quickly,which I couldn't think of, so went freezing instead (since it looks line if like polymerizing? And I think lots of people have seen those videos showing off that trick). If you want clear, beautiful ice, you've gotta freeze it the right way. But if you want to know if water is cold enough to freeze and don't care about using the ice, bump the supercooled bottle. Trying to convey the differences in constraints between a) detecting dna, where it's a destructive test for all intents and purposes since the end product is just tossed into biohazard - you just wants signal and b) making more usable dna for further research, where you want that end product for further manipulation and so has to high quality, usable dna, not just a polymerized mess. Lots of rapid PCR diagnostics take advantage of this to use gunk-creating rapid tests while scientists working on engineering the same DNA will use temperature cycling processes to clone the DNA and do work on it - each has its use.