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General description of Mologic/Sherlock Bioscience technology: "Mologic said it will invest a part of an expansion to its $4.9m grant from the Bill and Melinda
by pdm55 7y ago
General description of Mologic/Sherlock Bioscience technology:
"Mologic said it will invest a part of an expansion to its $4.9m grant from the Bill and Melinda-Gates Foundation to finance access to Sherlock's INSPECTR™ platform, which the Cambridge-based US company licences earlier this year from the Wyss Institute.
Under the agreement, Mologic will provide its lateral flow-based platform CARD, which has been demonstrated to provide a 1,000-fold improvement in sensitivity over current technology, and its immunoassay platform ELTABA for enzyme activity detection. Sherlock Bioscience will provide its synthetic biology-based INSPECTR™ platform, which consists of a DNA hybridization-based sensor that can be easily programmed to detect target nucleic acids (DNA or RNA) with single base pair specificity, coupled with a paper-based synthetic gene network that translates the sensor’s detection into a bioluminescent signal that is easily visualised or captured on instant film. Crucially, this process can be done at room temperature and does not require any instrumentation."
https://european-biotechnology.com/up-to-date/latest-news/news/sherlock-bioscience-and-mologic-enter-partnership.html https://european-biotechnology.com/up-to-date/latest-news/ne...
- pdm55 7y ago"What is SHERLOCK™? SHERLOCK is an evolution of CRISPR technology, which others use to make precise edits in genetic code. SHERLOCK can detect the unique genetic fingerprints of virtually any DNA or RNA sequence in any organism or pathogen. Developed by our founders and licensed exclusively from the Broad Institute, SHERLOCK is a method for single molecule detection of nucleic acid targets and stands for Specific High Sensitivity Enzymatic Reporter unLOCKing. It works by amplifying genetic sequences and programming a CRISPR molecule to detect the presence of a specific genetic signature in a sample, which can also be quantified. When it finds those signatures, the CRISPR enzyme is activated and releases a robust signal. This signal can be adapted to work on a simple paper strip test, in laboratory equipment, or to provide an electrochemical readout that can be read with a mobile phone." https://sherlock.bio/technology/ https://sherlock.bio/technology/
- pdm55 7y agoVideo of paper-based bioluminescence detection (Wyss Institute) "If the sample contains the RNA of the Zika virus, the test area turns purple." https://www.eurekalert.org/pub_releases/2016-05/uot-rld050616.php https://www.eurekalert.org/pub_releases/2016-05/uot-rld05061...
- pdm55 7y agoAn alternative: "Anitoa systems, a Silicon Valley biotech company has demonstrated a solution for rapid on-site nucleic acid test of the 2019 novel coronavirus (2019-nCoV). Anita combines a high performance ultra-portable Real Time Polymerase Chain Reaction (qPCR) instrument called Maverick, with an innovative “one-step” reverse transcription qPCR (RT-qPCR) reagent for testing 2019-nCoV." http://www.anitoa.com/docs/press-ncov.pdf http://www.anitoa.com/docs/press-ncov.pdf