Is the bit by bit construction of the protein an issue? I mean, surely the first half would have already folded into some shape by the time the second half was

Is the bit by bit construction of the protein an issue? I mean, surely the first half would have already folded into some shape by the time the second half was be added on? And then the rest of it would not necessarily fold the same way as if you considered the entire completed protein starting to fold from scratch.